(2 items) Adds 1096 Maximum Stamina (3 items) Adds 657 Critical Chance (4 items) Adds 657 Critical Chance (5 items) Adds 1528 Critical Chance For even more damage, and if you have access to a Trials Group you can replace Kinra with Arms Of Relequen. Alternative Setsįor alternatives, Leviathan or Tzogvin’s Warband can replace Kinra’s Wrath, though they will perform slighly lower in terms of overall DPS. This bonus increases by 143 every time Volley ticks, up to a maximum of 8 times. (2 items) Increases the damage Volley deals by 430 each tick. Set bonus (1 item) Adds 771 Critical Chance Removing Harpooner's Wading Kilt removes all stacks of Hunter's Focus. Taking direct damage removes 5 stacks of Hunter's Focus, up to once per second. Each stack of Hunter's Focus increases your Critical Chance by 125 and your Critical Damage by 1%. You can only gain 1 stack of Hunter's Focus per second. Set bonus (1 item) Dealing direct damage grants you a stack of Hunter's Focus for 1 minute, up to 10 stacks max. While you have an aura of wrath, allies within 12 meters of you gain Minor Berserk, increasing their damage done by 5%. While you have 5 stacks, you generate an aura of wrath, granting you Major Berserk, increasing your damage done by 10%. Set bonus (2 items) Adds 129 Weapon and Spell Damage (3 items) Adds 657 Critical Chance (4 items) Adds 129 Weapon and Spell Damage (5 items) Dealing damage with a Light or Heavy Attack grants you a stack of Burning Heart for 5 seconds, up to 5 stacks. Set bonus (2 items) Adds 129 Weapon and Spell Damage (3 items) Adds 657 Critical Chance (4 items) Adds 129 Weapon and Spell Damage (5 items) Increase the damage your Physical, Bleed, Poison, and Disease Damage over time and channeled abilities do by 15%. And to make the one remaining body piece, a Slimecraw should piece will be ideal thanks to the bonus Critical Chance. Of course the Thunderous Volley set adds greater DPS while utilizing the Volley skill on the back up (Two Bar version only). Next, the Harpooner’s Wading Kilt Mythic adds even more damage to the build in the form of lots of Critical Chance and bonus Critical Damage. This buffs our main spammable ability, Biting Jabs, by an amazing twenty percent!Ĭombined with this we are using Kinra’s Wrath which gives us passive Major Slayer for even more increased damage! This is one of the strongest sets for Stamina Damage dealers in the game, particularly if you don’t have access to Trials gear yet. Our main damage set on this build is the Deadly Strike set.
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Rather than adding this Steel String to the existing engine, RealGuitar is provided as two completely independent plug-ins: ‘Classic’ and ‘Steel String’. For those unfamiliar with MusicLab’s particular take on virtual guitars, the Basic Principles box provides a brief description.Ĭut-mix-dissolve to RealGuitar 5, which expands on the original set of acoustic guitars with a brand-new Steel String model. The fifth guitar in the series, RealEight (currently at version 1.0) was reviewed in the August 2015 edition of SOS. Version 4 improvements included simulated double tracking, customisable humanising (randomisation) of various parameters, and a built-in Song sequencer that enables the integral library of patterns and chords to be arranged as an entire song, constructed entirely within the plug-in and then dragged to the instrument’s DAW track as a MIDI file. RealGuitar developed in tandem with these, and by version 4 they all shared (by and large) the same set of features and functions. In the meantime we’ve reviewed other instruments in the series - RealStrat, RealLPC, RealRick. It was then at version 2, and has since passed through versions 3 and 4 and now arrived at 5. It was 2006 when we first reviewed MusicLab’s RealGuitar virtual instrument. MusicLab’s latest update makes RealGuitar more real than ever. Direct input (no ‘intelligent’ scripting), so the Classic remains backwards compatible with older projects. The ‘legacy’ Harmony, Chords, Bass’n’Chord and Bass’n’Pick modes are still here, along with all the original features. Last but not least, you can use the legato technique for playing notes with no intervening silence, and pick the performance mode (solo, harmony, bass and chord, bass and pick, and chords mode).ĭuring our testing we have noticed that the application provides excellent audio quality and without errors throughout the entire process.Īll things considered, RealGuitar comes packed with many useful features for helping you create guitar accompaniment tracks in a professional way.The Classic instrument in Solo mode. Plus, you can configure the audio parameters in terms of tremolo and chorus, user the MIDI keyboard to play chord changes, synchronize with the host’s tempo, beats, loop and time signature, drag and drop the selected audio pattern to your host’s MIDI track, perform major or minor triads, and adjust the number of strings. Other important features worth mentioning enable users to activate the auto fret position, as well as alter the accent velocity, samples, strum and release time, noises (fret, release and pick values), and sounds (mute, slow strum). RealGuitar provides different modes for playing the guitar, such as steel fingered, steel stereo, nylon fingered, steel stereo, and others.įurthermore, you can alter the master volume, use the built-in equalizer, change the pitch and modulation, adjust the tempo, as well as select the MIDI channels, buffer size, and bitrate. This way, you can simulate basic guitar techniques, such as strumming, plucking, sliding, bending, and muting. The program sports a clean interface that gives users the possibility to create musical compositions by playing to a virtual guitar. RealGuitar is an audio synthesizer designed to help you create, alter, or play guitar sounds. Be sure to set a positive tone when resigning You can explain this reasoning to your supervisor and tell them how your current position has helped you gain the relevant experience for the new role. End your explanation by discussing how your current position has prepared you for future opportunities.įor example, you are a customer service agent for a department store, and you accepted an offer to work as a customer service agent for a technology company, which is a goal you have been working toward. Consider talking about your career goals to keep the conversation professional. When you resign, give an honest and concise explanation for your decision. Secondly, explain why you plan to leave your current job No matter which approach you choose, try to schedule the meeting in advance so your supervisor has enough time to talk with you. If distance or scheduling make an in-person conversation challenging, request a phone call instead. Giving your notice in-person is the most respectful approach. If possible, plan to give your two weeks’ notice in person. Try to tell your direct manager first because you will already have an existing relationship with them. Firstly, schedule a time to talk to your manager or HR departmentĭepending on the size of your company, you may need to inform multiple people, including your manager, department head and the human resources department about your resignation. Here are the steps you should follow to give two weeks’ notice to your employer: 1. How to give two weeks’ notice to an employer During this time, you can train a new employee to perform your duties, finish any projects and notify any clients you regularly work with. Two weeks is the standard time for a resignation announcement in most industries. Giving two weeks’ notice means informing your manager you intend to leave your job in 14 days. Your resignation letter will stay in your employee file so human resources can keep a record of your employment dates. Many companies require that you submit your two weeks’ notice in writing, which is called a resignation letter. This article shows you how to give your two weeks’ notice with an example of a formal notice letter. Giving two weeks’ notice can be intimidating but it is important to show you care about your job and employer. Giving this notice is important because it leaves your manager with a positive impression and helps you maintain connections with your team. You should know how to give your two weeks’ notice to your manager if you are planning to leave your current job. How to give two weeks’ notice to an employer.What does giving two weeks’ notice mean?. Head-On expanded on this idea, changing the mask to face paint and the smile to his own. Starting with Twisted Metal: Black, his design became a large, somewhat overweight bare chested clown with a flaming mask locked into a permanent maniacal smile. Despite the changes some factors remained uniform, notably the perpetual flame on his head introduced in the second game. The character has gone through several redesigns differing from game to game, similar to fellow character Calypso, and his personality has grown progressively darker along the way. He is also directly responsible for the creation of that game's Axel, whose wife he killed, and Cage, who wishes to be a greater killer than Sweet Tooth. He has an unnamed brother that only appeared in Twisted Metal: Black, who drove that game's Yellow Jacket vehicle. Spectre's ending in the first game strongly hints that Sweet Tooth was the serial killer who killed the driver of Spectre five years before the competition. The final level of the second game features a boss fight with his father as Dark Tooth. Sweet Tooth has the most relationships to any group of characters in the entire series: his father Charlie Kane (driver of Yellow Jacket in the first game) and Marcus Kane (split personality, Roadkill's driver). He is the only character (besides Marcus Kane) to drive more than one vehicle in any of the games, being the driver of Head-On's Dark Tooth, Tower Tooth, and as of Twisted Metal: Lost, Gold Tooth. While being in every title of the series, he has not always been immediately available, requiring to be unlocked in some. Sweet Tooth is designed around the premise of a killer clown that drives a combat ice cream truck, and his face has been featured on the cover of every Twisted Metal game with the exception of Twisted Metal 2, making him the series' mascot. Sweet Tooth, is a vehicle driven by Marcus "Needles" Kane, who is a fictional character from the Twisted Metal video game series. Gilbert ( Twisted Metal: Black, Twisted Metal, PlayStation All-Stars Battle Royale)įred Tatasciore ( Twisted Metal: Head-On) Sweet Tooth in PlayStation All-Stars Battte Royale He gave Harry some important clues to identify the real monster inside the chamber of secrets, provided they could survive an encounter with his children. Aragog produces deadly venom and reaches up to 15 feet long, but it’s otherwise quite intelligent and even capable of speaking human languages. Ron and Harry meet Aragog, Hagrid’s blind acromantula, on the edge of the Forbidden Forest, where he lives with thousands of his creepy, crawly children. This giant eight-legged magical spider is supposedly native to the rainforests in the Pacific island of Borneo. Many of the animals in Harry Potter tend to be exaggerated forms of real animals. #7: Spiders Jumping spiders have incredible hearing. Because the eyes always remain in a fixed position, the owl must turn its head (up to 270 degrees) to see around it. While the bird only measures up to 27 inches tall, the yellow eyes are just as large as a human’s, which makes them proportionally huge in comparison to the head. The male is clad in all-white feathers, whereas the females and juveniles also have dark stripes or spots. In real-life, the snowy owl is a large, majestic bird that hails from the northern Arctic. Harry’s companion and pet owl, Hedwig, is one of the most well-known animals in Harry Potter who was given to him by Hagrid on his 11th birthday, proves her loyalty continuously throughout the entire series. In the wizarding world, snowy owls are the ultimate postmasters: they possess the ability to deliver mail even without knowing the address of the recipient. #8: Owls The snowy owl is also known as the polar owl. The revelation of Scabber’s true identity would continue to have important ramifications throughout the rest of the series. An animagus is a witch or wizard who can transform into an animal at will. First obtained from Ron’s older brother, Percy, Scabbers was later revealed to be none other than the animagus form of Peter Pettigrew, who was in hiding from the dark lord himself. Characterized by a ragged left ear and an amputated finger, Scabbers may be a type of agouti rat, at least judging by the fur color. Ron Weasley’s pet rat, Scabbers, is a bit of a lazy lout who’s constantly napping. Instead, it was a pure invention of future Academy Award-winning director Alfonso Cuaron, who wanted to create a magical scene for “Harry Potter and the Prisoner of Azkaban.” #9: Rats Rat tails help keep them cool by expanding and contracting the blood vessels to regulate body temperature. Book readers probably know that the frog choir did not make an appearance anywhere in J.K. They can be first seen performing “Something Wicked This Way Comes” during the Welcoming Feast after the Sorting Ceremony. Led by Professor Flitwick, the Frog Choir is a group of Hogwarts students who hold their talented toads aloft on pillows. One of the most memorable scenes in the Harry Potter series involves a duet between humans and singing toads. #10: Toads Groups of toads, such as the Frog Choir, are called knots. It will contain very minor spoilers, but this shouldn’t be a problem for anyone who has already read the books and seen the movies. This article will cover some interesting details about the top 10 animals in Harry Potter. Some of the concepts from the book, including the animagus and the Patronus, are based upon real animals in the normal “Muggle” world. Rowling’s wizarding world, which was first introduced with the release of “Harry Potter and the Philosopher’s Stone” in 1997, draws heavily on both historical mythology and the natural world to create its magical creatures. Someone is just about to hurt your friend.Meet Every Dinosaur Featured in Jurassic World….The one that lets you keep your honor the best.Horrible magical war number 2 has broken out, only this time which is good and which is bad is not so clear.Blindly attack without any reservations.Your best friend has just insulted you.A tendancy to be too devoted to one person.What word would you choose to describe yourself?.Which of these assets would you rather have?. The directive is required for instantiating the service, copying inbox binaries, and registering a device interface GUID that applications require to find the device and talk to it. To load the driver, you need to write an INF that references the modem INF ( mdmcpq.inf) by using the Include directive. In Windows 8.1 and earlier versions of the operating system, Usbser.sys is not automatically loaded when a USB-to-serial device is attached to a computer. With the new in-box INF, Usbser.sys is automatically loaded if a USB-to-serial device is detected on the mobile device. Unlike desktop editions, it is not possible to load a driver through an external driver package. On mobile editions of Windows, such as Windows 10 Mobile, only drivers that are part of the operating system are loaded. Microsoft encourages you to use in-box drivers whenever possible. For information about INF sections, see Overview of INF Files. For examples, look through the INF files included with sample drivers and find devices similar to your device. If your device specifies class and subclass codes to 02, and you want to load another driver instead of Usbser.sys, you have to write an INF that specifies the hardware ID of the device and the driver to install.In this case, you might have to load your own driver or write an INF that references another in-box driver. If a suitable driver is not found, the device might not have a driver loaded. The Plug and Play manager tries to find a driver. If your device specifies class code 02 but a subclass code value other than 02, Usbser.sys does not load automatically.With this approach, you are not required to distribute INF files for your device because the system uses Usbser.inf. For more information, see USB communications device class. If you want to load Usbser.sys automatically, set the class code to 02 and subclass code to 02 in the Device Descriptor.The driver is loaded based on a compatible ID match similar to other USB device class drivers included in Windows. You do not need to write your own INF to reference the driver. If your device belongs to the communications and CDC control device class, Usbser.sys is loaded automatically. Starting in Windows 10, Usbser.inf was added to the %Systemroot%\INF directory, which loads Usbser.sys as the functional device object (FDO) in the device stack. For the list of USB device class driver included in Windows, see USB device class drivers included in Windows. If they are not installed automatically, contact the device manufacturer. If you trying to install a USB device class driver included in Windows, you do not need to download the driver. The shade range is somewhat limited, but if you can find a good match, this is a great product for those of us who’d rather skip foundation and use something that’s lighter. My skin absolutely looks better and it doesn’t feel heavy on the skin at all. I also opted to finish off with a damp beauty blender to ensure there was no streaking and so that the product settled nicely into the skin.Īfter multiple uses, I’m loving the results. I did feel like it dried fairly quickly, so you may want to keep that in mind. I pumped a small amount of product to the back of my hand, tapped my brush into it, and then swept onto my face. I’ve been using Radiant Defense (shade: Beige) quite a bit since receiving it and I love that it combines skincare and makeup benefits.Īt first use, I was surprised at the level of coverage it provided, which was really nice, and the Radiant Defense Liquid brush made it easy and quick to apply. There are six shades available: Shell, Beige, Sand, Golden, Almond and Espresso. Since it’s tinted, it does provide some lightweight coverage to help even skin tone and blur pores. With an SPF of 30, it also touts pretty decent protection against UVA/UVB rays. The lightweight, oil-free formulation provides three forms of defense: environmental, barrier and photo defense. Radiant Defense is a new skin tint that protects your skin against the sun and other stressors while improving your complexion over time. So when Rodan and Fields recently launched their Radiant Defense Perfecting Liquid Broad Spectrum SPF 30, I had to give it a try. All opinions are my own.I love natural-looking skin and given that I struggle with hyperpigmentation and sun damage, having a tinted moisturizer/CC cream with SPF is a must. I have never before used a foundation that can make those claims! Radiant Defense Perfecting Liquid leaves my skin in better condition than ever. I am thrilled that it contains SPF 30, because I do not need to wear a separate sunscreen product, and my skin in protected and ready to go. It covers all of my imperfections and makes my complexion look flawless. It looks and feels like a second skin, and yet does not look like I have makeup on my face. It leaves a beautiful, radiant finish that never looks shiny or oily. It enhances my skin, and gives me a youthful, healthy glow. (You can also, apply it with your foundation brush or sponge.) I love the light, non-greasy formula that is so easy to blend. I love how you have the option to select your matching shade by uploading a photo of yourself to virtually try on different shades of Radiant Defense to find your best match. From their six available shades, I discovered my perfect skin tone match, which is the Sand shade.įrom the moment I first applied Radiant Defense Perfecting Liquid to my skin, I could tell that it was going to become my new favorite foundation.Īfter cleansing in the morning, I apply my Perfecting Liquid to my entire face, neck and upper chest area, and blend it gently with my fingertips. Trust your skin to the doctors who’ve gotten it from the get glow.” It helps neutralize the harmful effects of environmental aggressors, repair skin’s natural moisture barrier and protect it from sun damage caused by UVA and UVB rays. Why do we call Radiant Defense a Glow Guard? Its unique and proprietary RD3 Complex protects your skin. Use as the last step of your skincare routine to protect and perfect the glowing skin you’ve earned. Formulated with dermatology-inspired skincare ingredients to deliver a radiant, healthy-looking complexion in six flexible shades. “Introducing our new, groundbreaking tinted Glow Guard, Radiant Defense Perfecting Liquid Broad Spectrum SPF 30. I wear foundation every day no matter what, and when I learned that Rodan + Fields launched their Radiant Defense Perfecting Liquid, I couldn’t wait to get the chance to try it for myself. Their new Radiant Defense Perfecting Liquid is truly amazing! I was very excited to find out that Rodan + Fields came out with a new product. I love every single one of their products that I have tried. One of the very best skin care companies I have ever experienced is Rodan+ Fields. He lives with and cooks for his wife and son in San Antonio. He's a Houston native with philosophy degree from Harvard and a master's from the Columbia Graduate School of Journalism. Anthony Hotel, Rebelle has completely revamped its menu and it is making waves. He began his newspaper career in Bay City, Texas, before going to the Corpus Christi Caller-Times, graduate school in New York, a yearlong internship at the Chicago Tribune and different positions at the Milwaukee Journal and Milwaukee Journal Sentinel. The Finest Seafood Restaurant in San Antonio, TX. His other assignments for the paper included education and news obituaries. Before joining the Taste team, he wrote the ""Around the Town"" column for the Express-News form 2002-2008. He joined the Express-News in 1999 after closing his restaurant. He is a frequent contributor to and has written about food, restaurants and reviewing for Epicurious and Romenesko. His culinary explorations began with rolling flour tortillas as a child and included a yearlong stint as chef and owner of a restaurant in Milwaukee. Please note a 50% of service fee is applicable to all same-day cancellations and no shows.Edmund Tijerina writes about food and reviews restaurants for the San Antonio Express-News. All communication between ourselves and clients is done in this manner and is essential to the way we operate our business. You must be able to send and receive texts to book with us. Sorry, but we do not do razor fades, "edgars", design work, beard fills, or across the forehead edge-ups, as these styles are not part of our skill-sets. REBELLE, San Antonio - Menu, Prices & Restaurant Reviews - Tripadvisor Rebelle Claimed Save Share 223 reviews 74 of 3,007 Restaurants in San Antonio Vegetarian Friendly Vegan Options Gluten Free Options 300 E Travis St The St. We do combovers, undercuts, shags/moptops, men's long hair, fauxhawks, slickbacks, pompadours, high n tights and fades as low as #00. Anthony Hotel, 21, On the menu: Divide (lighter shared plates) 10-17 Conquer (heavier dishes) 25. We're a specialty shop focusing ONLY on styles that can be reasonably executed in 30 minutes or less. Only serving his existing clientele, no new clients please. First-timers please book with her, she's awesome. Our event-deck's offer numerous culinary options that can suite your party's needs, be it private or corporate, with a knowledgeable, friendly and professional staff lead by local San Antonio celebrity Chef Stefan Bowers. She's serving the needs of all new folks. Rebelle offers multiple private dinning options perfect for any occasion. Tina - Barbering stylist, fresh in town from Austin. Mon: subject to weekly availability, check for booking openings With cult classics on the flatscreen, great vibes on the smartspeaker, and cool hosts, Rebel Rebel is the place you've been looking for. Experience fine dining surrounded by ornate wooden staircases, Italian marble bars and funky chandeliers. Every client gets a full 30 minutes to themselves, with all the extras like hot steam towel, nape shave and classic shoulder massage machine. Seafood San Antonio chef Stefan Bowers creates masterful seafood dishes at the award-winning restaurant ReBelle, located inside our luxury hotel. It's an "adult swim" kinda place, specially designed for men and boys 13/up to relax, have a cold drink and forget your troubles. We are the go-to place for guys who not only need a great cut, but some down time as well. If you need a barber, please book on this app. Had great expectations for an upscale dinner experience, but found a lackluster experience instead. Anthony Hotel, 300 E Travis St in San Antonio - view the menu, hours, phone number, address and map. Prices are based on an average dinner, per person. Located in the former ballroom of the St Anthony Hotel, Rebelles dark walls. It took two: Tastes of the Merguez goat kebabs and the Brussels sprouts in crème fraiche laden with hints of wine, dill and orange zest. Latest reviews, photos and ratings for Rebelle at The St. On : Review: Reborn Rebelle now San Antonio’s finest seafood restaurant under 15 / 16-30 / 31-50 / over 50. From traditional to trendy, these 15 San Antonio restaurants are as. Anthony Hotel, Rebelle has become my example of see how amazing San Antonio’s dining scene is and I knew I could turn this guy around in three bites. If you need product, come on in and shop our growing selection, no appointment required. Since opening last fall in the revamped St. We're a small, 2-chair barber studio/ men's grooming products store with a "dive bar" feel. We tried about 5 main course dishes, all were cooked & seasoned. Howdy, partner! Welcome to Rebel Rebel Men's Grooming. Read 5 tips and reviews from 264 visitors about fancy, desserts and brunch food. We are leaving our Booksy page active temporarily until everyone gets moved over to the new platform. IMPORTANT UPDATE: WE ARE NO LONGER USING BOOKSY FOR APPOINTMENTS. ( Does not include Arrests by County Sheriff, State Police, or Surrounding Municipalities). Criminal History Requests Requests for Lincoln Police Department public criminal history information. Arrests made by Chicago Police Department. Police Records City of Lincoln, NE Home / City Information / Departments / Police / Police Records Police Police Records By Phrase or Keyword 10 Result (s) Found Accident Reports Accident reports completed by the Lincoln Police Department.Adults (18 and over) at time of arrest.Police Reports Taken by the San Diego Police Department Includes requests for arrests, crimes, and event incident reports Here are the ways to obtain a report: Mail Send your request and a stamped, self-addressed envelope to: SDPD Records MS726 P.O. For information concerning the expungement process, visit: Police Records & Procedures Records are limited to: For instructions on how to get a police report, select the type of report below. If expunged, the appropriate record(s) are removed. All named offenders are presumed innocent unless and until proven guilty in a court of law. The Internet Criminal History Access Tool (ICHAT) allows the search of public criminal history record information maintained by the Michigan State Police. This site is made available for the use and benefit of law enforcement partners, news media, and members of the public to search Chicago Police public arrest records including Name, Mugshot, Age, Address, Central Booking Number, Charges, Arrest Date/Time, Arrest Location, Date Time Released from Chicago Police Facility, Bond Type/Amount/Date, and the geographic police area/district/beat.Īrrest records provided on this public website or through its interface pertain to individuals who have been charged with a crime as an adult. Some reports are only available online while requests for Certificates of Conduct must be made in person at Police Headquarters in Lower Manhattan. This is Chicago Police Department official website for searching arrest records. The departments Certificate of Conduct and Non-Criminal Fingerprint Section, Criminal Records Section, and Aided Unit provide different types of records or reports upon request. Characteristics of coherent gating technique are inherently well-suited for ideal histopathologic imaging, and it is particularity useful as an alternative to histological tissue observation when it is not practical to take specimens for histological processing or when large areas of tissue are needed to be investigated. In particular, tomographic imaging has an important role as optical biopsy, because it can provide a real-time structural information non-invasively at spatial resolutions similar to that of a standard histological section taken from a biopsy sample. To date, optical imaging based on coherent gating technique has widely been applied in the clinic to diagnose diseases of various tissues 27, 28. OCM is also suitable for deep tissue imaging, because OCM uses near infrared light that reaches deeper into biological tissues than visible light can reach. Thus, it does not require chemical labeling, staining or external contrast agents. OCM is based on a coherent gating technique of optical interferometry, and utilizes the endogenous back-scattering signal. Optical coherence microscopy (OCM) 22, 23, 24, 25, 26 could be an alternative imaging modality for high throughput histopathology. An ideal 3D optical imaging technique for histopathology would be able to image unlabeled and unstained fresh specimens and provide high throughput quantitative information as well as native 3D context. However, it requires careful sample preparation for clearing protocols as well as long incubation for immunostaining, which can often distort the physical volume of the entire 3D specimens and thus can restrict its utility for pathology. This approach allows fast volumetric imaging without the need for mechanical sectioning. A number of tissue clearing techniques 14, 19 have been introduced and successfully combined with light-sheet microscopy to unveil the cellular connectivity at large areas. As the effective clearing agents are available, the deep tissue optical imaging has become feasible through either the refractive index matching of tissue or removal of the highly scattered biological components like lipid. While these techniques can generate a complete dataset such as projectomes and connectomes, the high cost of equipment, the huge amount of time needed for tissue processing and fluorescent labeling, and massive data collection and signal processing make this technique difficult to be widely available for pathology applications.Īnother novel approach for volumetric imaging is based on light-sheet fluorescence microscopy and chemically clearing tissue 14, 15, 16, 17, 18, 19, 20, 21. One of representative attempts of fully automated bright field microscopy 11, single photon microscopy 12 and two-photon microscopy combined with tissue sectioning method 13 were successfully used to reconstruct fine neuronal network and anatomical tracings in a whole mouse brain. 3D microscopic imaging techniques have been initially applied to neuroscience and developmental studies. Over the past decade, advanced 3D microscopic imaging techniques for high resolution and volumetric anatomy have been demonstrated. However, current methods lack the ability to visualize diagnostic pathologic feature over large areas or volumetric context. In order to overcome current restriction of histopathology, several optical imaging techniques have been introduced toward rapid and stain-free histopathology of fresh tissues 4, 5, 6, 7, 8, 9, 10. Although histologic analysis in pathology is a gold-standard to understand tissue morphology, diagnose diseases, and decide treatment course, it is still very time- and labor-intensive and provides only limited tissue information in thin and narrow field of view. The histological examination of excised tissue via optical microscope has been widely used in biological laboratory and clinic, which requires the cumbersome process such as fixation, embedding, sectioning, and staining of biological specimens 1, 2, 3. |
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